High Performance Liquid Chromatography (HPLC) Practice Exam

The Certificate in High Performance Liquid Chromatography (HPLC) provides Candidates with a thorough understanding of the principles, techniques, and applications of HPLC. Candidates learn about the components of an HPLC system, chromatographic theory, column selection, and method development. The course covers topics such as sample preparation, mobile phase selection, and data analysis.

The certification covers skills in HPLC method development, troubleshooting, data interpretation, and instrument maintenance. Candidates also learn about regulatory compliance and quality assurance in HPLC.

Candidates should have a basic understanding of chemistry and analytical techniques. Previous experience with chromatography can be beneficial but is not required.
Why is High performance liquid chromatography (HPLC) important?

• Essential for separating, identifying, and quantifying components in a mixture
• Plays a key role in pharmaceutical analysis, environmental testing, and food analysis
• Critical for quality control and assurance in various industries
• Important for research and development in chemistry and biology

Who should take the High performance liquid chromatography (HPLC) Exam?

• Analytical Chemist
• Quality Control Analyst
• Research Scientist
• Pharmaceutical Scientist
• Environmental Analyst

Skills Evaluated

Candidates taking the certification exam on the High performance liquid chromatography (HPLC) is evaluated for the following skills:

• HPLC Method Development
• Troubleshooting
• Data Interpretation
• Instrument Maintenance
• Knowledge of Regulatory Compliance

High performance liquid chromatography (HPLC) Certification Course Outline

• Introduction to HPLC
  • Principles of Chromatography
  • Components of an HPLC System
• HPLC Method Development
  • Column Selection
  • Mobile Phase Selection
• Sample Preparation for HPLC
  • Extraction Techniques
  • Filtration and Degassing
• Data Analysis in HPLC
  • Peak Integration
  • Calibration Curves
• Troubleshooting in HPLC
  • Common Issues and Solutions
  • Maintenance of HPLC Systems